A spatio-temporal larval distribution model of the nuisance midge Glyptotendipes paripes Edwards (Diptera: Chironomidae) was used to design resource efficient sampling plans for the assessment of location(s) supporting nuisance population levels of this species in Lake Monroe (central Florida, USA). The model used bathymetric and sediment maps with lake water levels and temperatures of the prior month to estimate lake areas supporting relatively low larval populations (<100/m²) that require minimal monitoring effort, and areas that potentially support nuisance levels of G. paripes larvae, requiring greater sampling resources. The advantage of this system is that the geographic stratification can be altered for each sampling effort to meet prevailing conditions in the lake and without relying on a generic stratification that may not match the existing lake situation.